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37 .   Isolation of potential novel cellulolytic and xylanolytic bacteria and optimization of their cultural conditions for enhanced production of cellulase and xylanase

Authors: DivyaTandon, Nivedita Sharma

Volume 20, Issue 3;
Pages: 231-240;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551



Abstract:

The aim of the present study is to demonstrate potential cellulolytic as well as hyperxylanolytic bacteria and optimization of culture conditions for enhanced enzyme activity. Cellulolytic and xylanolytic bacteria were isolated from diverse sources viz; soil, sludge, rice fields etc. by standard serial dilution and pour plate method. Bacteria were further identified by morphological and biochemical tests and subjected to enzyme production. Twenty three different bacterial strains were isolated and screened for cellulase and xylanase production in submerged fermentation process. Among them, two tested bacterial strains; R2, R4 showed maximum yield for cellulase production. While SD9 showed higher xylanase production. This strain was further characterized by biochemical and morphological as well as genotypic identification and identified as Bacillus licheniformis R2, Bacillus mojavensis R4. Xylanoytic bacteria was identified as Bacillus atropheaus SD9. Results indicated that favourable conditions and the selection of a suitable growth medium played a key role in the production of enzyme from newly isolated Bacillus licheniformis R2, Bacillus mojavensis. Xylanoytic bacteria was identified as Bacillus atropheaus SD9. Due to its particular characteristics this enzyme will be used in scarification process for bioethanol production from plant biomasses.

Keywords: Cellulase, Xylanase, Bacillus, CMC agar

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