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2021 Journal of Agroalimentary Processes and Technologies 2021, 27(3)
 
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12 .   High potential sources of resistance to spot blotch in barley supported by AFLP markers

Authors: A. Aldaoude *, M. Jawhar, E. Al-Shehadah, A. Shoaib, M.I.E. Arabi

Volume 27, Issue 1;
Pages: 74-80; 2021
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551



Abstract:

Spot blotch (SB) caused by Cochliobolus sativus presents a serious problem for barley production worldwide, and identification new sources of resistance is a key objective for many breeders. Breeding for resistance is an economically and environmentally friendly approach to reduce negative effects of the disease, and understanding the genetic basis of this resistance can enhance the development of resistant varieties. With that goal, F2 barley recombinant lines obtained from a cross of highly barley resistant cv. Banteng and the susceptible cv. WI2291 were tested for SB resistance which supported by AFLP markers. Following greenhouse evaluations under SB artificial infection conditions, results demonstrated different significant levels among barley recombinant lines ranged from highly susceptible to resistant. However, out of the 54 recombinant lines, 25 were resistant than the others which had 7 AFLP unique bands at different regions in the genome, this distribution and resistance or susceptibility can indicate that some positive or negative markers are common either to resistant or susceptible lines. The unique and common AFLP bands might be of considerable interest for enhancing effective resistance to SB.

Keywords: Barley, spot blotch, recombinant lines, AFLP polymorphism, resistance

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