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2019 Journal of Agroalimentary Processes and Technologies 2019, 25(4)
 
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62 .   Assessment of genetic variation in accessions of sesame (Sesamum indicum L.) and its crosses by seed protein electrophoresis

Authors: Musibau A. Azeez, Charity O. Aremu, Oyinkansola O. Olaniyan

Volume 19, Issue 4;
Pages: 383-391;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551



Abstract:

Seed storage proteins of seventeen accessions and seven crosses of sesame were analysed using polyacrylamide gel electrophoresis with a view to assessvariation. A total of 19 protein polypeptide bands were detected with relative migration of 0.13 to 0.87. The maximum number of protein bands (12) was recorded in EVAx69B-882 and the minimum (6) in 69B-882xPACH, 69B-882xEVA, C-K21xPACHcrosses and E-8 accession. Band number 1 and 13 were exclusive to 65-8B while 5 to 69B-882xC-K21. Each genotype was distinct from the other, but certain bands were shared by several genotypes. Principal component analysis of protein data revealed the first three principal components accounting for 57.08% of the total variability. The dendrogram generated from cluster analysis grouped the accessions and the crossesinto four distinct clusters. This study reveals moderate genetic variability among the genotypes from various sources and suggests possibilities for improvementthrough breeding.

Keywords: SDS-PAGE, polypeptide bands, genetic variability, cluster analysis, crosses, Sesame

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