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53 .   Phenolic Content and Antioxidant Activity in Milling Fractions of Oat

Authors: Cristian P.Serea, Octavian Barna

Volume 17, Issue 3;
Pages: 291-294;
ISSN: 2069-0053 (print) (former: ISSN:1453-1399), Agroprint;
ISSN (online):2068 – 9551



Abstract:

The aim of the research is to study the antioxidant activity and concentration of phenolic substances with antioxidant role in milling fractions of two characteristic cultivars of Romanian oat. The method Folin – Ciocâlteu has been used to determine the total content of phenolic substances. The total antioxidant activity was determined by spectrophotometric method using the reagent DPPH (2,2-Diphenyl-1-Picryl Hydrasil), as system of releasing the free radical DPPH. The antioxidant activity is expressed as antiradical activity of samples and as percentage. Both cultivars analyzed have registered the highest content of phenolic substances in bran, and the antioxidant capacity of the fractions analyzed has been strongly correlated with the content of phenolic substances and that of ash. The data resulted from the experiment confirm the positioning of substances with antioxidant role especially in the superior strata of oat grain and high quantity of substances with antioxidant role in oat.

Keywords: phenolic content, Antioxidant activity, oat, milling fractions

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