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28 .   Quantitative determination of caffeine in carbonated beverages by an HPLC method

Authors: Nour Violeta, Trandafir I., Ionica Mira Elena


Abstract:

The purpose of this work was to adapt and use the HPLC method proposed by SR EN 12856/2001 standard for the determination of some artificial sweeteners (acesulfame-K, aspartame and saccharin) for the quantification, in a single run, of the caffeine too. The method was validated in terms of sensitivity, linearity range, reproducibility, repeatability, analytical recovery and robustness. In this paper we present the results obtained by our laboratory in two proficiency testing schemes, the first organized by FAPAS, Central Science Laboratory, Sand Hutton, York, United Kingdom and the second organized by Food and Consumer Product Safety Authority (Chek Working Group), Al Groningen, The Netherlands.

Keywords: caffeine, HPLC, diode array detection, carbonated beverages.

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