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2019 Journal of Agroalimentary Processes and Technologies 2019, 25(4)
 
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25 .   Monitoring Superoxide Dismutase Activity in freshwater crustacean organisms exposed to the environmental metallotoxin Pb(II). The case of Daphnia magna

Authors: P. Peppos, A. Salifoglou, S. Hadjispyrou

Volume 18, Issue 2;
Pages: 116-119;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551



Abstract:

The goal of this work was the study of the toxic action of well-defined forms of the environmental metallotoxin Pb(II) on marine organisms known for their presence in the environment. To this end, the binary Pb(II)–(Heida) complex was investigated on the freshwater index-organism Daphnia magna. The results obtained from the toxicity experiments have been processed through the “Trevors” program, in order to assess the toxicity of the Pb(II), expressed as EC50-48h. The results suggest an EC50-48h value of 4.46 mg/L, leading to the conclusion that the specific form of Pb(II) is toxic to Daphnia magna. Subsequently, the influence of Pb(II) on the activity of the antioxidant enzyme Cu,Zn-SOD, isolated properly from the organism, was carried out. From the results of this study, it was found that the Pb(II) complex has practically no influence on the specific enzyme activity.

Keywords: Lead ecotoxicity, Daphnia magna, Cu,Zn-SOD enzyme activity, 2-hydroxyethyl-iminodiacetic Pb(II), EC50-48h

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